Efficient gene targeting in the moss Physcomitrella patens
نویسندگان
چکیده
منابع مشابه
Gene targeting in Physcomitrella patens.
Gene-targeting efficiency in the land plant Physcomitrella patens (Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae. Sequencing programs and microbiological molecular genetic approaches are now being developed to unravel the precise function of plant genes. Physcomitrella patens, as the new 'green yeast', might well become a major tool for functional genomic studie...
متن کاملCulturing the moss Physcomitrella patens.
This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by se...
متن کاملRNA interference in the moss Physcomitrella patens.
The moss Physcomitrella patens performs efficient homologous recombination, which allows for the study of individual gene function by generating gene disruptions. Yet, if the gene of study is essential, gene disruptions cannot be isolated in the predominantly haploid P. patens. Additionally, disruption of a gene does not always generate observable phenotypes due to redundant functions from rela...
متن کاملParameters determining the efficiency of gene targeting in the moss Physcomitrella patens
In the moss Physcomitrella patens, transforming DNA containing homologous sequences integrates predominantly by homologous recombination with its genomic target. A systematic investigation of the parameters that determine gene targeting efficiency shows a direct relationship between homology length and targeting frequency for replacement vectors (a selectable marker flanked by homologous DNA). ...
متن کاملMRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens
The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DN...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1997
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1997.11061195.x